These Hela cells were prepared for undergraduate students to learn how to take digital images on fluorescence microscopes and to overlay the images into different color channels. We were also discussing and observing the changes that take place to the cytoskeleton (tubulin and actin) and DNA (DAPI) during the various phases of mitosis that were present in the preparation.
ProgRes® C3 cooled camera with CapturePro V2.5.
The microscope was a Zeiss AxioImager A1, with an X-Cite light source. Filter sets are DAPI, FITC, RHODAMINE, the objective a 40x/0.75 EC-PlanNeo Fluar. The computer was a Mac.